演讲摘要：

Highly accurate 3D measurements of refractive index (RI) distribution in phase microstructures are becoming increasingly important for quality control in industry and quantitative label-free imaging of cells and tissues in biomedical applications. The measurement tool fulfilling most of the requirements for these applications is optical diffraction tomography based on holographic projections. In the talk, at first, the principles of full and limited projection angle holographic tomography (HT) are presented. Next the recent advances in architectures of tomographic systems, numerical approaches andreconstruction algorithms are discussed. Special attention is given to novel schemes of modulation of an object illumination beam, numerical focusing, aberration compensation and holographic data manipulation for both full (FAHT) and limited projection angle tomography (LAHT). New measurement scenarios and algorithms for limited angle tomographic reconstruction based on generalized total variation iterative constraint strategy and extension of depth-of-field are presented. Novel 3D phase phantom for metrological validation of research and commercial holographic tomographs is introduced and the results of comparison between HT systems using different architectures and reconstruction algorithms is presented. Multiple interesting examples of FAHT applications including measurements offibre optics accessories (microtips/ microbridges) and microlenses are presented. High applicability of LAHT in biomedicine at cellular levelis demonstrated through measurements and monitoring of numerous cells and cell cultures subjected to different environmental changes. Finally the challenges connected with the future development of holographic tomography scanners for digital phase pathology are discussed.